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bio gel htp hydroxyapatite  (Bio-Rad)


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    Structured Review

    Bio-Rad bio gel htp hydroxyapatite
    Bio Gel Htp Hydroxyapatite, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 742 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hydroxylapatite+bio+gel+htp+gel/Bio-Gel+HTP+Hydroxyapatite/us12612440-2295-48-51
    Average 94 stars, based on 742 article reviews
    bio gel htp hydroxyapatite - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Chromatography:

    Article Title: Compositions and methods for purifying calreticulin
    Article Snippet: .. Lysate Buffer (L-Buffer) 150 mM NaCl 100 mM KH2PO4, pH-7.1 2 mM MgCl2 Q Sepharose Chromatography Column Buffers Column details: HiLoad 26/10 Fast Flow column was used from GE Healthcare Bio-Sciences cat #17-1062-01 Q Sepharose Binding buffer (BBQ) 150 mM NaCl 100 mM KH2PO4, pH-7.1 2 mM MgCl2 Q Sepharose Elution buffer (EBQ) 1000 mM NaCl 100 mM KH2PO4, pH-7.1 2 mM MgCl2 Hydroxylapatite Chromatography Column Buffers Column details: Hydroxylapatite Bio-gel HTP Gel, Bio-Rad cat#130-0420 Binding buffer (BBH) 10 mM K2HPO4, pH-7.0 Wash buffer (WBH) (use 2 column volumes for washing) 80 mM K2HPO4, pH-7.0 Elution buffer (EBH) (for continuous salt gradient from 80-1000 mM) 80 mM K2HPO4, pH-7.0 1000 mM K2HPO4, pH-7.0 Resource Iso (Isopropyl) Chromatography Column Buffers Column details: RESOURCE ISO 1 ml column from GE Healthcare Bio-Sciences cat #17-1185-01 Binding buffer (BBI) Buffers: 40% Ammonium Sulfate in 10 mM K2HPO4, pH-7.2 Elution buffer (EBI) 10 mM K2HPO4, pH-7.2 Protein storage Buffer 10 mM Tris-HCl, pH-7.0 1 mM EDTA The protein may also be stored in calcium containing buffers as long as calcium concentration does not exceed 10 mM. ..

    Binding Assay:

    Article Title: Compositions and methods for purifying calreticulin
    Article Snippet: .. Lysate Buffer (L-Buffer) 150 mM NaCl 100 mM KH2PO4, pH-7.1 2 mM MgCl2 Q Sepharose Chromatography Column Buffers Column details: HiLoad 26/10 Fast Flow column was used from GE Healthcare Bio-Sciences cat #17-1062-01 Q Sepharose Binding buffer (BBQ) 150 mM NaCl 100 mM KH2PO4, pH-7.1 2 mM MgCl2 Q Sepharose Elution buffer (EBQ) 1000 mM NaCl 100 mM KH2PO4, pH-7.1 2 mM MgCl2 Hydroxylapatite Chromatography Column Buffers Column details: Hydroxylapatite Bio-gel HTP Gel, Bio-Rad cat#130-0420 Binding buffer (BBH) 10 mM K2HPO4, pH-7.0 Wash buffer (WBH) (use 2 column volumes for washing) 80 mM K2HPO4, pH-7.0 Elution buffer (EBH) (for continuous salt gradient from 80-1000 mM) 80 mM K2HPO4, pH-7.0 1000 mM K2HPO4, pH-7.0 Resource Iso (Isopropyl) Chromatography Column Buffers Column details: RESOURCE ISO 1 ml column from GE Healthcare Bio-Sciences cat #17-1185-01 Binding buffer (BBI) Buffers: 40% Ammonium Sulfate in 10 mM K2HPO4, pH-7.2 Elution buffer (EBI) 10 mM K2HPO4, pH-7.2 Protein storage Buffer 10 mM Tris-HCl, pH-7.0 1 mM EDTA The protein may also be stored in calcium containing buffers as long as calcium concentration does not exceed 10 mM. ..

    Concentration Assay:

    Article Title: Compositions and methods for purifying calreticulin
    Article Snippet: .. Lysate Buffer (L-Buffer) 150 mM NaCl 100 mM KH2PO4, pH-7.1 2 mM MgCl2 Q Sepharose Chromatography Column Buffers Column details: HiLoad 26/10 Fast Flow column was used from GE Healthcare Bio-Sciences cat #17-1062-01 Q Sepharose Binding buffer (BBQ) 150 mM NaCl 100 mM KH2PO4, pH-7.1 2 mM MgCl2 Q Sepharose Elution buffer (EBQ) 1000 mM NaCl 100 mM KH2PO4, pH-7.1 2 mM MgCl2 Hydroxylapatite Chromatography Column Buffers Column details: Hydroxylapatite Bio-gel HTP Gel, Bio-Rad cat#130-0420 Binding buffer (BBH) 10 mM K2HPO4, pH-7.0 Wash buffer (WBH) (use 2 column volumes for washing) 80 mM K2HPO4, pH-7.0 Elution buffer (EBH) (for continuous salt gradient from 80-1000 mM) 80 mM K2HPO4, pH-7.0 1000 mM K2HPO4, pH-7.0 Resource Iso (Isopropyl) Chromatography Column Buffers Column details: RESOURCE ISO 1 ml column from GE Healthcare Bio-Sciences cat #17-1185-01 Binding buffer (BBI) Buffers: 40% Ammonium Sulfate in 10 mM K2HPO4, pH-7.2 Elution buffer (EBI) 10 mM K2HPO4, pH-7.2 Protein storage Buffer 10 mM Tris-HCl, pH-7.0 1 mM EDTA The protein may also be stored in calcium containing buffers as long as calcium concentration does not exceed 10 mM. ..

    other:

    Article Title: Penicillium purpurogenum produces a highly stable endo-β-(1,4)-galactanase.
    Article Snippet: The polysaccharides of galactose present in the pectin of the plant cell wall are degraded by endo-β-1,4-galactanases.. The filamentous fungus Penicillium purpurogenum, which grows on a number of natural carbon sources, among them sugar beet pulp which contains pectin, has a gene (ppgal1) coding an endo-β-1,4-galactanase (PpGAL1).. This enzyme was expressed heterologously in Pichia pastoris.



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